TNFRSF8
(Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
蛋白类型
Recombinant
产品特性
AA 19-379
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF8 protein is labelled with His-Avi Tag.
原理
Human CD30/TNFRSF8 Protein
序列
Phe19-Lys379
产品特性
Recombinant Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD30, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail.
TNFRSF8
宿主: 人
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
TNFRSF8
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % by SDS-PAGE gel analyses
WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF8
(Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
CD30 Protein, D1S166E Protein, Ki-1 Protein, Cd30 Protein, Ki Protein, TNFRSF8 Protein, TNF receptor superfamily member 8 Protein, tumor necrosis factor receptor superfamily, member 8 Protein, TNFRSF8 Protein, Tnfrsf8 Protein
背景
The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
分子量
41.3 kDa. Due to glycosylation, the protein migrates to 68-95 kDa based on Tris-Bis PAGE result.