TNFSF8 Protein (AA 63-234) (mFc Tag)
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- 抗原 See all TNFSF8 蛋白
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- 蛋白类型
- Recombinant
- 产品特性
- AA 63-234
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This TNFSF8 protein is labelled with mFc Tag.
- 原理
- Human CD30 Ligand/TNFSF8 Protein
- 序列
- Gln63-Asp234
- 产品特性
- Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Gln63-Asp234.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC,
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD30, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (His tag)
TNFSF8 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) protein (mFc-His Tag)TNFSF8 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (His-DYKDDDDK-Strep II Tag)TNFSF8 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) (Active) protein (His tag)TNFSF8 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) proteinTNFSF8 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 1-234) protein (GST tag)TNFSF8 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 153-234) protein (GST tag)TNFSF8 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 1-234) protein (Strep Tag)Crystallography grade TNFSF8 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 1-239) protein (Strep Tag)Crystallography grade TNFSF8 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (His tag)Crystallography grade TNFSF8 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- 别名
- CD30 Ligand (TNFSF8 产品)
- 别名
- CD153 Protein, CD30LG Protein, Cd30l Protein, CD30L Protein, TNFRSF8 Protein, TNFSF8 Protein, tumor necrosis factor (ligand) superfamily, member 8 Protein, TNF superfamily member 8 Protein, tumor necrosis factor superfamily member 8 Protein, Tnfsf8 Protein, TNFSF8 Protein
- 背景
- CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
- 分子量
- 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
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