This CD3 epsilon protein is labelled with Fc-Avi Tag,Biotin.
原理
Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein
序列
Asp22-Thr48
产品特性
Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp22-Thr48.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CD3 Antibody, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27 peptide, hFc Tag with the EC50 of 6ng/ml determined by ELISA. See testing image for detail.
CD3E
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
分子量
31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.