CD3 epsilon Protein (CD3E) (AA 23-126) (His tag,AVI tag,Biotin)
(1 reference)-
- 抗原 See all CD3 epsilon (CD3E) 蛋白
- CD3 epsilon (CD3E)
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-126
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD3 epsilon protein is labelled with His tag,AVI tag,Biotin.
- 序列
- AA 23-126
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product CD3E 蛋白
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CD3 epsilon (CD3E) protein (Fc Tag)
CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) protein (His tag)CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) protein (His tag)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 23-126) protein (Fc Tag)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag)CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag,Biotin)CD3E 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag,Biotin)CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (Active) protein (mFc Tag)CD3E 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD3 epsilon (CD3E) (AA 23-126) protein (His tag)CD3E 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
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: "Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA." in: SLAS discovery : advancing life sciences R & D, Vol. 25, Issue 9, pp. 977-984, (2020) (PubMed).
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: "Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA." in: SLAS discovery : advancing life sciences R & D, Vol. 25, Issue 9, pp. 977-984, (2020) (PubMed).
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3 epsilon (CD3E 产品)
- 背景
- CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
- 分子量
- 15.5 kDa
- NCBI登录号
- NP_000724
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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