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CD3E & CD3G (AA 22-117) protein (Fc Tag)

宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7274132
发货至: 中国
  • 抗原
    CD3E & CD3G
    蛋白类型
    Recombinant
    产品特性
    AA 22-117
    宿主
    • 7
    • 4
    • 3
    • 2
    • 1
    Cynomolgus
    资源
    • 17
    HEK-293 Cells
    标记
    Fc Tag
    原理
    Cynomolgus CD3E & CD3G/CD3 epsilon & CD3 gamma Protein
    序列
    Gln22-Asp117 (CD3E) & Gln23-Thr113 (CD3G)
    特异性
    Uni-Prot: Q95LI5.2 (CD3E), Q95LI7 (CD3G)
    产品特性
    Recombinant Cynomolgus CD3E & CD3G/CD3 epsilon & CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G).
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with EC50 of 51.2ng/ml determined by ELISA. See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    CD3E & CD3G
    别名
    CD3E&CD3G
    背景
    T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
    分子量
    36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
    UniProt
    Q95LI5
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