CD3E & CD3G (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag)
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- 抗原
- CD3E & CD3G
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-126
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- His tag,Fc Tag,DYKDDDDK Tag
- 应用范围
- Functional Studies (Func)
- 序列
- AA 23-126
- 产品特性
- The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
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- 抗原
- CD3E & CD3G
- 背景
- T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
- 分子量
- 39.2 kDa (CD3E) & 38.6 kDa (CD3G)
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