CD3D & CD3E (AA 22-117) protein (Fc Tag)
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- 抗原
- CD3D & CD3E
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-117
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宿主
- Cynomolgus
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资源
- HEK-293 Cells
- 标记
- Fc Tag
- 原理
- Cynomolgus CD3E & CD3D/CD3 epsilon & CD3 delta Protein
- 序列
- Gln22-Asp117 (CD3E) & Phe22-Ala105 (CD3D)
- 特异性
- Uni-Prot: Q95LI5.2 (CD3E), Q95LI8 (CD3D)
- 产品特性
- Recombinant Cynomolgus CD3E & CD3D/CD3 epsilon & CD3 delta Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D).
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, mFc Tag with EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.
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CD3D & CD3E (AA 23-126) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3D & CD3E protein (Fc-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-120) protein (His tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-108) protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-126) (Active) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (Fc Tag,His tag,DYKDDDDK Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD3D & CD3E
- 别名
- CD3E&CD3D
- 别名
- AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein
- 背景
- T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- 分子量
- 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa (CD3E) and 44-46 kDa (CD3D) based on Tris-Bis PAGE result.
- UniProt
- Q95LI5
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