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CD3D & CD3E (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag,Biotin)

宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
产品编号 ABIN2870530
发货至: 中国
  • 抗原
    CD3D & CD3E
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 23-126
    宿主
    • 8
    • 7
    • 4
    • 2
    • 2
    资源
    • 22
    • 1
    HEK-293 Cells
    标记
    His tag,Fc Tag,DYKDDDDK Tag,Biotin
    品牌
    MABSol®,UltraLys
    序列
    AA 23-126
    特异性
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
    产品特性
    The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3D carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag. The protein has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . The protein migrates as 40-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
    纯度
    >95 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
  • 说明

    A chemically labeled biotinylated protein with ultra sensitivity.
    The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
    Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • 抗原
    CD3D & CD3E
    别名
    CD3E & CD3D
    别名
    AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein
    背景
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
    分子量
    39.2 kDa (CD3E), 37.5 kDa (CD3D)
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