CD20 (MS4A1)
(Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
蛋白类型
Recombinant
产品特性
AA 141-188
宿主
人
资源
大肠杆菌(E. Coli)
标记
This CD20 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human CD20/MS4A1 Protein
序列
Ile141-Ser188
产品特性
Recombinant Biotinylated Human CD20/MS4A1 Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Ile141-Ser188.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human CD20, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 87.3ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
MS4A1
宿主: 人
宿主: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as 0.22μm filtered solution in 20 mM PB, 10 % Glycerol ( pH 7.0).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
CD20 (MS4A1)
(Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.