CA9 Protein (AA 38-414) (His-Avi Tag)
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- 抗原 See all CA9 蛋白
- CA9 (Carbonic Anhydrase IX (CA9))
- 蛋白类型
- Recombinant
- 产品特性
- AA 38-414
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CA9 protein is labelled with His-Avi Tag.
- 原理
- Human CA9/Carbonic Anhydrase IX Protein
- 序列
- Gln38-Asp414
- 产品特性
- Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln38-Asp414.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CA9, His Tag at 0.9μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CA9 蛋白
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Carbonic Anhydrase IX (CA9) (AA 38-402) protein (His tag)
CA9 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase IX (CA9) (AA 32-390) protein (His tag)CA9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase IX (CA9) (AA 1-459) protein (GST tag)CA9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase IX (CA9) (AA 38-153) protein (His-IF2DI Tag)CA9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Carbonic Anhydrase IX (CA9) protein (His tag)CA9 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Carbonic Anhydrase IX (CA9) (Active) protein (His tag)CA9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Carbonic Anhydrase IX (CA9) protein (His tag,Biotin)CA9 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Carbonic Anhydrase IX (CA9) (AA 38-414) protein (His tag)Crystallography grade CA9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase IX (CA9) (AA 32-390) protein (His tag)Crystallography grade CA9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase IX (CA9) (AA 38-459) protein (rho-1D4 tag)Crystallography grade CA9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CA9 (Carbonic Anhydrase IX (CA9))
- 别名
- CA9 (CA9 产品)
- 别名
- CA9 Protein, CAIX Protein, Ca9 Protein, MN/CA9 Protein, MN Protein, carbonic anhydrase 9 Protein, CA9 Protein, CAH9 Protein, Car9 Protein
- 背景
- CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
- 分子量
- 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q16790
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