BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
蛋白类型
Recombinant
产品特性
Trimer
宿主
人
资源
HEK-293 Cells
标记
This BAFF protein is labelled with His-Avi Tag,Biotin.
序列
Thr141-Leu285
纯度
> 95% as determined by Tris-Bis PAGE,> 94% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human BAFFR, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human BAFF (Trimer) , His Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail.
TNFSF13B
宿主: 人
宿主: Tobacco (Nicotiana benthamiana)
Recombinant
> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
WB, CC
Active
TNFSF13B
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
BAFF, BLyS, CD257, TNFSF13B, TNFSF20, DTL, TALL1, TALL-1delta BAFF, TALL1Delta4 BAFF, THANK, ZTNF4, TALL-1,B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
分子量
54.2 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.