Recombinant Human ADAM8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Pro655.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human ADAM8, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD156 Protein, MS2 Protein, CD156a Protein, E430039A18Rik Protein, adam8 Protein, reprolysin Protein, si:zc217g15.7 Protein, zgc:64059 Protein, ADAM8 Protein, RGD1566394 Protein, ADAM metallopeptidase domain 8 Protein, a disintegrin and metalloproteinase domain 8 Protein, a disintegrin and metallopeptidase domain 8 Protein, ADAM metallopeptidase domain 8a Protein, ADAM8 Protein, LOC100304737 Protein, Adam8 Protein, adam8a Protein, adam8 Protein
背景
A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions. Their protumorigenic potential has been attributed to both, essential (ADAM10 and ADAM17) and 'dispensable' ADAM proteases (ADAM8, 9, 12, 15, and 19). Of specific interest in this review is the ADAM proteinase ADAM8 that has been identified as a significant player in aggressive malignancies including breast, pancreatic, and brain cancer.