UBE2W Protein (His tag)
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- 抗原 See all UBE2W 蛋白
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This UBE2W protein is labelled with His tag.
- 原理
- Recombinant Human UBE2W Protein (His Tag)
- 序列
- Met 1-Cys 151
- 产品特性
- A DNA sequence encoding the human UBE2W (Q96B02-12) (Met 1-Cys 151) was expressed, with a polyhistidine tag at the N-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product UBE2W 蛋白
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Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (His tag)
UBE2W 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-162) protein (His tag)UBE2W 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-159) protein (GST tag)UBE2W 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 17-112) protein (GST tag)UBE2W 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (Strep Tag)Crystallography grade UBE2W 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151), (full length) protein (His-SUMO Tag)UBE2W 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2W 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2W 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 100 mM Arg.0.1 % Brij35, pH 8.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- 别名
- UBE2W (UBE2W 产品)
- 别名
- UBE2W Protein, im:6905459 Protein, si:ch211-193n21.4 Protein, ube2wa Protein, UBC-16 Protein, UBC16 Protein, 6130401J04Rik Protein, ubiquitin conjugating enzyme E2 W Protein, ubiquitin-conjugating enzyme E2W (putative) Protein, ubiquitin conjugating enzyme E2 W (putative) Protein, ubiquitin conjugating enzyme E2W (putative) Protein, ubiquitin-conjugating enzyme E2W Protein, UBE2W Protein, ube2w Protein, Ube2w Protein
- 背景
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Background: Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.
Synonym: UBC-16;UBC16
- 分子量
- 19.2 kDa
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