UBE2W Protein (AA 1-151, full length) (His-SUMO Tag)
-
- 抗原 See all UBE2W 蛋白
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- 蛋白类型
- Recombinant
- 产品特性
- full length, AA 1-151
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This UBE2W protein is labelled with His-SUMO Tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product UBE2W 蛋白
-
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (His tag)
UBE2W 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-162) protein (His tag)UBE2W 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-159) protein (GST tag)UBE2W 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 17-112) protein (GST tag)UBE2W 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (Strep Tag)Crystallography grade UBE2W 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2W 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2W 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
- 抗原
- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
- 别名
- UBE2W (UBE2W 产品)
- 别名
- UBE2W Protein, im:6905459 Protein, si:ch211-193n21.4 Protein, ube2wa Protein, UBC-16 Protein, UBC16 Protein, 6130401J04Rik Protein, ubiquitin conjugating enzyme E2 W Protein, ubiquitin-conjugating enzyme E2W (putative) Protein, ubiquitin conjugating enzyme E2 W (putative) Protein, ubiquitin conjugating enzyme E2W (putative) Protein, ubiquitin-conjugating enzyme E2W Protein, UBE2W Protein, ube2w Protein, Ube2w Protein
- 背景
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi ania complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.
- 分子量
- 33.3 kDa
- UniProt
- Q96B02
-
