USP7 Protein (AA 208-560) (GST tag,His tag)
-
- 抗原 See all USP7 蛋白
- USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))
- 蛋白类型
- Recombinant
- 产品特性
- AA 208-560
- 宿主
- 人
-
资源
- Baculovirus infected Insect Cells
- 标记
- This USP7 protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human USP7/HAUSP Protein (aa 208-560, His & GST Tag)
- 序列
- Lys 208-Glu 560
- 产品特性
- A DNA sequence encoding the amino acid sequence (Lys 208-Glu 560) of human USP7 (NP_003461.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 98 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product USP7 蛋白
-
Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 214-521) protein (His tag)
USP7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 13-110) protein (GST tag)USP7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 1-1102) protein (Strep Tag)Crystallography grade USP7 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) protein (Myc-DYKDDDDK Tag)USP7 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))
- 别名
- USP7/HAUSP (USP7 产品)
- 别名
- CG1490 Protein, Dmel\\CG1490 Protein, USP7 Protein, 2210010O09Rik Protein, AA409944 Protein, AA617399 Protein, AU019296 Protein, AW548146 Protein, C80752 Protein, Hausp Protein, HAUSP Protein, TEF1 Protein, UBP Protein, Ubiquitin-specific protease 7 Protein, ubiquitin carboxyl-terminal hydrolase 7 Protein, ubiquitin specific peptidase 7 Protein, Usp7 Protein, LOC100201254 Protein, USP7 Protein
- 背景
-
Background: Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein which belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 stimulates lytic infection and the reactivation of quiescent viral genomes. ICP0 interacts very strongly with USP7. USP7-mediated stabilization of ICP0 is dominant over ICP0-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP0-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP0 are expressed.
Synonym: HAUSP;TEF1
- 分子量
- 68.8 kDa
- NCBI登录号
- NP_003461
-
