USP5 Protein (His tag)
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- 抗原 See all USP5 蛋白
- USP5 (Ubiquitin Specific Peptidase 5 (USP5))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This USP5 protein is labelled with His tag.
- 原理
- Recombinant Human USP5/ISOT Protein (His Tag)
- 序列
- Met 1-Ser 835
- 产品特性
- A DNA sequence encoding the human USP5 isoform short (P45974-2) (Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 92 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product USP5 蛋白
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Ubiquitin Specific Peptidase 5 (USP5) (AA 163-291) protein (His tag)
USP5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin Specific Peptidase 5 (USP5) (AA 1-858) protein (GST tag)USP5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin Specific Peptidase 5 (USP5) (AA 71-180) protein (GST tag)USP5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin Specific Peptidase 5 (USP5) (AA 2-858) protein (His tag)Crystallography grade USP5 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Ubiquitin Specific Peptidase 5 (USP5) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)USP5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- USP5 (Ubiquitin Specific Peptidase 5 (USP5))
- 别名
- USP5/ISOT (USP5 产品)
- 别名
- ISOT Protein, AA407472 Protein, ISOT-1 Protein, Ucht Protein, ubiquitin specific peptidase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5, putative Protein, ubiquitin specific protease 5 Protein, ubiquitin specific peptidase 5 (isopeptidase T) Protein, USP5 Protein, CNH02890 Protein, EHI_049940 Protein, CC1G_12961 Protein, MCYG_01862 Protein, CGB_L3600C Protein, PY00546 Protein, Usp5 Protein
- 背景
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Background: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Synonym: ISOT
- 分子量
- 94.7 kDa
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