TNF alpha Protein (AA 89-235)
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北京 101111
Quick Overview for TNF alpha Protein (AA 89-235) (ABIN7320677)
抗原
See all TNF alpha 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 89-235
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原理
- Recombinant Mouse TNF-alpha/TNFA Protein
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序列
- Asp89-Leu235
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产品特性
- Recombinant Mouse Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml.
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin,His-Avi Tag)
TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 78-233) protein (His tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His-Avi Tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag)TNF alpha 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor alpha (TNF alpha) (AA 78-235) (Active) proteinTNF alpha 宿主: 兔 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. CC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) (Active) protein (His tag)TNF alpha 宿主: 大鼠 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Tumor Necrosis Factor alpha (TNF alpha) protein (His tag)TNF alpha 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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别名
- TNF-alpha/TNFA
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背景
- Cachectin,DIF,Membrane Form,TNF-Alpha,TNF-a,TNF-alpha,TNFSF2,TNFalpha,Tnf,Tnfa,Tnfsf1a,Tnfsf2,Tumor Necrosis Factor,Tumor Necrosis Factor Ligand Superfamily Member 2,Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
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分子量
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Calculated MW: 16.4 kDa
Observed MW: 14 kDa
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基因ID
- 21926
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UniProt
- P06804
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途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
抗原
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