DIABLO Protein (His tag)
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- 抗原 See all DIABLO 蛋白
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This DIABLO protein is labelled with His tag.
- 原理
- Recombinant Human SMAC/Diablo Protein (His Tag)(Active)
- 序列
- Ala 56-Asp 239
- 产品特性
- A DNA sequence encoding the mitochondrial-located form of human Diablo (NP_063940.1) (Ala 56-Asp 239) was expressed with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized recombinant human SMAC-His at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg/ml.
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- Discover our top product DIABLO 蛋白
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Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 61-239) protein (His-IF2DI Tag)
DIABLO 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239) protein (T7 tag)DIABLO 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 56-239) proteinDIABLO 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 119-218) protein (GST tag)DIABLO 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 1-239) protein (His-GST)DIABLO 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA
Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 54-237) protein (His tag)Crystallography grade DIABLO 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Second Mitochondria-Derived Activator of Caspase (DIABLO) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DIABLO 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 别名
- SMAC/Diablo (DIABLO 产品)
- 别名
- smac Protein, smac3 Protein, diablo-s Protein, 0610041G12Rik Protein, 1700006L01Rik Protein, AU040403 Protein, Smac Protein, DFNA64 Protein, SMAC Protein, DBO Protein, XSmac Protein, diablo, IAP-binding mitochondrial protein Protein, diablo IAP-binding mitochondrial protein Protein, diablo, IAP-binding mitochondrial protein L homeolog Protein, diablo Protein, Diablo Protein, DIABLO Protein, diablo.L Protein
- 背景
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Background: Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.
Synonym: DFNA64,SMAC
- 分子量
- 22 kDa
- NCBI登录号
- NP_063940
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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