NEDD8 Protein (SUMO Tag,His tag)
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- 抗原 See all NEDD8 蛋白
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NEDD8 protein is labelled with SUMO Tag,His tag.
- 原理
- Recombinant Human NEDD8 Protein (His Tag, SUMO Tag)
- 序列
- Met 1-Gly76
- 产品特性
- Recombinant Human Neural precursor cell expressed developmentally down-regulated protein 8 is produced by our E.coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product NEDD8 蛋白
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Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) protein
NEDD8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) protein (GST tag)NEDD8 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-76) protein (His tag)Crystallography grade NEDD8 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl,5 % Trehalose, pH 7.4.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 别名
- NEDD8 (NEDD8 产品)
- 别名
- NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein
- 背景
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Background: Human NEDD8 shares 60 % amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Synonym: Neural precursor cell expressed developmentally down-regulated protein 8, NEDD8, Neddylin, Ubiquitin-like protein Nedd8,
- 分子量
- 20.9 kDa
- UniProt
- Q15843
- 途径
- Ubiquitin Proteasome Pathway
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