NEDD8 Protein (AA 1-76) (His tag,SUMO Tag)
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- 抗原 See all NEDD8 蛋白
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-76
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NEDD8 protein is labelled with His tag,SUMO Tag.
- 原理
- Recombinant Human NEDD8 (N-6His, SUMO tag)
- 序列
- MGHHHHHHGS LQDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME AFAKRQGKEM DSLRFLYDGI RIQADQAPED LDMEDNDIIE AHREQIGGML IKVKTLTGKE IEIDIEPTDK VERIKERVEE KEGIPPQQQR LIYSGKQMND EKTAADYKIL GGSVLHLVLA LRGG
- 产品特性
- Recombinant Human Neural precursor cell expressed developmentally down-regulated protein 8/NEDD8 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gly76) of Human NEDD8 fused with a 6His tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NEDD8 蛋白
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Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) protein
NEDD8 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) protein (GST tag)NEDD8 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-76) protein (His tag)Crystallography grade NEDD8 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl,5 % Trehalose, pH 7.4.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 5 months
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- 抗原
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 别名
- NEDD8 (NEDD8 产品)
- 别名
- NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein
- Sub Type
- Fusionprotein
- 背景
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Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Alternative Names: Neural precursor cell expressed developmentally down-regulated protein 8, NEDD8, Neddylin, Ubiquitin-like protein Nedd8, - 分子量
- 20.9 kDa
- UniProt
- Q15843
- 途径
- Ubiquitin Proteasome Pathway
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