Intestinal Alkaline Phosphatase Protein (ALPI) (His tag)
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- 抗原 See all Intestinal Alkaline Phosphatase (ALPI) 蛋白
- Intestinal Alkaline Phosphatase (ALPI)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Intestinal Alkaline Phosphatase protein is labelled with His tag.
- 原理
- Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active)
- 序列
- Met 1-Asp 503
- 产品特性
- A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 1,000 pmoles/min/μg.
- Top Product
- Discover our top product ALPI 蛋白
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Intestinal Alkaline Phosphatase (ALPI) (AA 74-162) protein (GST tag)
ALPI 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Intestinal Alkaline Phosphatase (ALPI) protein (Myc-DYKDDDDK Tag)ALPI 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Intestinal Alkaline Phosphatase (ALPI)
- 别名
- Alkaline Phosphatase/ALPI (ALPI 产品)
- 别名
- IAP Protein, Alp1 Protein, S51097 Protein, 2010001C14Rik Protein, Akp6 Protein, gIAP Protein, alpi Protein, im:7149588 Protein, si:ch211-262h13.1 Protein, wu:fa95f04 Protein, zgc:110794 Protein, ALPI Protein, Alpi Protein, alkaline phosphatase, intestinal Protein, alkaline phosphatase, intestinal, tandem duplicate 1 Protein, intestinal-type alkaline phosphatase Protein, ALPI Protein, Alpi Protein, alpi.1 Protein, LOC100724954 Protein
- 背景
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Background: ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Synonym: IAP
- 分子量
- 54 kDa
- UniProt
- P09923
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