Interleukin 35 Protein (IL35) (Fc Tag)
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- 抗原 See all Interleukin 35 (IL35) 蛋白
- Interleukin 35 (IL35)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Interleukin 35 protein is labelled with Fc Tag.
- 原理
- Recombinant Human Interleukin-35/IL-35 Protein (Fc Tag)(Active)
- 序列
- Met 1-Lys 229
- 产品特性
- A DNA sequence encoding the human IL35 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of human IL12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
- 纯度
- > 85 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to bind biotinylated human IL6RB-Fch in a functional ELISA.
- Top Product
- Discover our top product IL35 蛋白
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Interleukin 35 (IL35) (AA 21-229), (AA 23-219) (Active) protein (His tag)
IL35 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Interleukin 35 (IL35)
- 别名
- Interleukin-35/IL-35 (IL35 产品)
- 背景
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Background: The novel Ebi3-IL-12alpha heterodimeric cytokine has been designated interleukin-35 (IL-35); is a member IL12 family cytokine produced by regulatory T cells (Treg); but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of IL-12α (P35) and IL-27β chains; which are encoded by two separate genes called IL12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells; whereas recombinant IL-35 suppresses T-cell proliferation. It identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells; suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4(+)CD25(+)Foxp3(+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation; another well-known anti-inflammatory cytokine; along with TGFbeta cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo; IL-35 effectively attenuated established collagen-induced arthritis in mice; with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus; IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development.
Synonym: CLMF;IL-12A;IL-35;Interleukin-35;NFSK;NKSF1;P35
- 分子量
- 73.4 kDa
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