ICAM-3/CD50 Protein (His tag,Fc Tag)
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- 抗原 See all ICAM-3/CD50 (ICAM3) 蛋白
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ICAM-3/CD50 protein is labelled with His tag,Fc Tag.
- 原理
- Recombinant Human CD50/ICAM-3 Protein (His & Fc Tag)(Active)
- 序列
- Met 1-His 485
- 产品特性
- The extracellular domain (Met 1-His 485) of human ICAM3 (NP_002153.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3-coated plates (12.5 μg/ml, 100 μl/well), approximately 45%-60% will adhere specifically.
- Top Product
- Discover our top product ICAM3 蛋白
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Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-485) protein (His tag)
ICAM3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) protein (GST tag)ICAM3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 别名
- CD50/ICAM-3 (ICAM3 产品)
- 别名
- CD50 Protein, CDW50 Protein, ICAM-R Protein, ICAM-3 Protein, ICAM3 Protein, intercellular adhesion molecule 3 Protein, ICAM3 Protein, Icam3 Protein
- 背景
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Background: The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 Molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.
Synonym: CD50,CDW50,ICAM-3,ICAM-R
- 分子量
- 77.2 kDa
- NCBI登录号
- NP_002153
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