HTRA2 Protein (His tag)
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- 抗原 See all HTRA2 蛋白
- HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This HTRA2 protein is labelled with His tag.
- 原理
- Recombinant Human HtrA2/Omi Protein (His Tag)(Active)
- 序列
- Ala 134-Glu 458
- 产品特性
- A DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) was expressed, with a polyhistide tag at the C-terminus.
- 纯度
- > 87 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.
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- Discover our top product HTRA2 蛋白
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HtrA Serine Peptidase 2 (HTRA2) (full length) protein (His tag)
HTRA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
HtrA Serine Peptidase 2 (HTRA2) (AA 133-358) protein (His tag)HTRA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
HtrA Serine Peptidase 2 (HTRA2) (AA 134-458) protein (His tag)HTRA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
HtrA Serine Peptidase 2 (HTRA2) (AA 359-458) protein (GST tag)HTRA2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
HtrA Serine Peptidase 2 (HTRA2) (AA 134-458) protein (rho-1D4 tag)Crystallography grade HTRA2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
HtrA Serine Peptidase 2 (HTRA2) (AA 134-458) protein (rho-1D4 tag)Crystallography grade HTRA2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 0.3M NaCl, 1 mM DTT, 20 % Glycerol, pH 7.8
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
- 别名
- HtrA2/Omi (HTRA2 产品)
- 别名
- CG8464 Protein, DmHtrA2 Protein, Dmel\\CG8464 Protein, HtrA2/Omi Protein, Omi Protein, dOmi Protein, domi Protein, htrA2 Protein, omi Protein, HTRA2 Protein, PRSS25 Protein, OMI Protein, PARK13 Protein, AI481710 Protein, Prss25 Protein, mnd2 Protein, HTRA2-related serine protease Protein, HtrA serine peptidase 2 Protein, HtrA2 Protein, HTRA2 Protein, htra2 Protein, Htra2 Protein
- 背景
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Background: Serine protease HTRA2, also known as high temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein which belongs to the peptidase S1B family. HTRA2 contains one PDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa.
Synonym: Serine protease HTRA2; mitochondrial;High temperature requirement protein A2;HtrA2;Omi stress-regulated endoprotease;Serine protease 25;Serine proteinase OMI;HTRA2;OMI; PRSS25
- 途径
- Positive Regulation of Endopeptidase Activity
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