Ephrin B1 Protein (EFNB1) (His tag,Fc Tag)
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- 抗原 See all Ephrin B1 (EFNB1) 蛋白
- Ephrin B1 (EFNB1)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Ephrin B1 protein is labelled with His tag,Fc Tag.
- 原理
- Recombinant Human Ephrin-B1/EFNB1 Protein (His & Fc Tag)(Active)
- 序列
- Met 1-Gly 232
- 产品特性
- A DNA sequence encoding the human EFNB1 (NP_004420.1) extracellular domain (Met 1-Gly 232) was was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
- 纯度
- >(79.7+18.0) % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 μg/ml (100 μl/well) can bind human EFNB1 Fc chimera with a linear ranger of 1.56-25 ng/ml.
- Top Product
- Discover our top product EFNB1 蛋白
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Ephrin B1 (EFNB1) (AA 28-237) protein (His tag)
Crystallography grade EFNB1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Ephrin B1 (EFNB1) protein (His tag,Fc Tag)EFNB1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Ephrin B1 (EFNB1) (AA 1-346) protein (GST tag)EFNB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ephrin B1 (EFNB1) (AA 118-217) protein (GST tag)EFNB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Ephrin B1 (EFNB1)
- 别名
- Ephrin-B1/EFNB1 (EFNB1 产品)
- 别名
- EFNB1 Protein, LERK2 Protein, Cek5-L Protein, EFL-3 Protein, Elk-L Protein, Epl2 Protein, Eplg2 Protein, LERK-2 Protein, Lerk2 Protein, Stra1 Protein, CFND Protein, CFNS Protein, EFL3 Protein, EPLG2 Protein, cfnd Protein, cfns Protein, efl3 Protein, efnb1-A Protein, ephrinB1 Protein, eplg2 Protein, lerk2 Protein, ephrin B1 Protein, ephrin-B1 Protein, ephrin-B1 L homeolog Protein, EFNB1 Protein, Efnb1 Protein, efnb1 Protein, efnb1.L Protein
- 背景
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Background: Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.
Synonym: Ephrin-B1,EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2,LERK-2,CFND,CFNS,EFB1,EFL3,Elk-L,EPLG2,LERK2
- 分子量
- 51.2 kDa
- NCBI登录号
- NP_004420
- 途径
- RTK signaling
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