DAP Kinase 1 Protein (AA 1-363) (GST tag,His tag)
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- 抗原 See all DAP Kinase 1 (DAPK1) 蛋白
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-363
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This DAP Kinase 1 protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human DAPK1/DAP Kinase 1 Protein (aa 1-363, His & GST Tag)(Active)
- 序列
- Met 1-Leu 363
- 产品特性
- A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 80 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
- Top Product
- Discover our top product DAPK1 蛋白
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Death-Associated Protein Kinase 1 (DAPK1) (AA 1-368) protein (GST tag)
DAPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 1 (DAPK1) (AA 1211-1310) protein (GST tag)DAPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 1 (DAPK1) protein (Myc-DYKDDDDK Tag)DAPK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
- 别名
- DAPK1/DAP Kinase 1 (DAPK1 产品)
- 别名
- DAPK1 Protein, MGC81366 Protein, si:ch211-66i11.1 Protein, wu:fj09c03 Protein, dapk Protein, MGC83745 Protein, 2310039H24Rik Protein, 2810425C21Rik Protein, AI642003 Protein, D13Ucla1 Protein, DAP-Kinase Protein, DAPK Protein, death-associated protein kinase 1 Protein, death associated protein kinase 1 L homeolog Protein, death associated protein kinase 1 Protein, death associated protein kinase 1 S homeolog Protein, LOC427465 Protein, dapk1.L Protein, DAPK1 Protein, dapk1 Protein, dapk1.S Protein, Dapk1 Protein
- 背景
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Background: Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).
Synonym: DAPK
- 分子量
- 69.4 kDa
- 途径
- MAPK Pathway, Interferon-gamma Pathway
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