CLPS Protein (His tag)
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- 抗原 See all CLPS 蛋白
- CLPS (Colipase, Pancreatic (CLPS))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CLPS protein is labelled with His tag.
- 原理
- Recombinant Human CLPS/Colipase Protein (His Tag)(Active)
- 序列
- Met 1-Gln 112
- 产品特性
- A DNA sequence encoding the human CLPS (P04118) (Met 1-Gln 112) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized human CLPS-His at 10μg/mL(100μL/well) can bind biotinylated human PNLIP-His.The EC50 of biotinylated human PNLIP-His is 0.57-1.33μg/mL.
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- Discover our top product CLPS 蛋白
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Colipase, Pancreatic (CLPS) (AA 18-112) protein (His tag)
CLPS 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Colipase, Pancreatic (CLPS) (AA 23-112) protein (GST tag)CLPS 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Colipase, Pancreatic (CLPS) (AA 1-112) protein (GST tag)CLPS 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Colipase, Pancreatic (CLPS) (AA 23-112) protein (His tag)Crystallography grade CLPS 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Colipase, Pancreatic (CLPS) (AA 24-113) protein (His tag)Crystallography grade CLPS 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Colipase, Pancreatic (CLPS) protein (Myc-DYKDDDDK Tag)CLPS 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0, 10 % gly
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CLPS (Colipase, Pancreatic (CLPS))
- 别名
- CLPS/Colipase (CLPS 产品)
- 别名
- CLPS Protein, 2200003J09Rik Protein, COLQ Protein, colipase Protein, colipase, pancreatic Protein, CLPS Protein, Clps Protein
- 背景
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Background: Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.
Synonym: CLPS
- 分子量
- 11.5 kDa
- UniProt
- P04118
- 途径
- Lipid Metabolism
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