Factor XI Protein (His tag)
-
- 抗原 See all Factor XI (F11) 蛋白
- Factor XI (F11) (Coagulation Factor XI (F11))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This Factor XI protein is labelled with His tag.
- 原理
- Recombinant Human Coagulation Factor XI/F11 Protein (His Tag)(Active)
- 序列
- Met 1-Val 625
- 产品特性
- A DNA sequence encoding the human F11 (NP_000119.1) precursor (Met 1-Val 625) with a carboxy-terminal polyhistidine tag was expressed.
- 纯度
- > 85 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
- Top Product
- Discover our top product F11 蛋白
-
Coagulation Factor XI (F11) (AA 19-625) protein (His tag)
F11 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Coagulation Factor XI (F11) (AA 1-625) protein (GST tag)F11 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Coagulation Factor XI (F11) (AA 286-385) protein (GST tag)F11 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Coagulation Factor XI (F11) (AA 19-387) protein (His tag)Crystallography grade F11 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Coagulation Factor XI (F11) (AA 388-625) protein (His tag)Crystallography grade F11 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Coagulation Factor XI (F11) (AA 19-389) protein (His tag)Crystallography grade F11 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Coagulation Factor XI (F11) (AA 390-624) protein (His tag)Crystallography grade F11 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- Factor XI (F11) (Coagulation Factor XI (F11))
- 别名
- Coagulation Factor XI/F11 (F11 产品)
- 别名
- FXI Protein, 1600027G01Rik Protein, AI503996 Protein, Cf11 Protein, LRRGT00086 Protein, coagulation factor XI Protein, F11 Protein, CpipJ_CPIJ000593 Protein, CpipJ_CPIJ001060 Protein, CpipJ_CPIJ002752 Protein, CpipJ_CPIJ003631 Protein, CpipJ_CPIJ004041 Protein, CpipJ_CPIJ004093 Protein
- 背景
-
Background: Factor XI (plasma thromboplastin antecedent) is a plasma glycoprotein, and a zymogen acting as a serine protease which participates in blood coagulation as a catalyst in the conversion of factor IX to factor IXa in the presence of calcium ions. It is an unusual dimeric protease, with structural features that distinguish it from vitamin K-dependent coagulation proteases. The factor XI is synthesized in the liver as a single polypeptide chain with a molecular weight estimated between 125 ~160 kDa and then is processed into a disulfide-bond linked homodimer. FXI is a homodimer, with each subunit containing four apple domains and a protease domain. The apple domains form a disk structure with binding sites for platelets, high molecular weight kininogen, and the substrate factor IX (FIX). FXI is converted to the active protease FXIa by cleavage of the Arg369-Ile370 bond on each subunit. After the activation reaction, Factor XIa is composed of two heavy and two light chains held together by three disulfide bonds. The heavy chains are derived from the amino termini of the zymogen and responsible for the binding of factor XI to high molecular weight kininogen and for the activation of factor IX, while the light chain contains the catalytic portion of the enzyme and is homologous to the trypsin family of serine proteases. FXI deficiency is a disorder characterized by a mild or no bleeding tendency. Severe FXI deficiency is an injury-related bleeding disorder common in Ashkenazi Jews and rare worldwide.
Synonym: coagulation factor 11,coagulation factor XI,FXI
- 分子量
- 69.5 kDa
- NCBI登录号
- NP_000119
-
