Cathepsin S Protein (CTSS) (His tag)
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- 抗原 See all Cathepsin S (CTSS) 蛋白
- Cathepsin S (CTSS)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Cathepsin S protein is labelled with His tag.
- 原理
- Recombinant Human Cathepsin S/CTSS Protein (His Tag)(Active)
- 序列
- Met 1-Ile 331
- 产品特性
- A DNA sequence encoding the pro form of human CTSS (NP_004070.3) (Met 1-Ile 331) with a carboxy-terminal polyhistidine tag was expressed.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl)-NH2 (R&D Systems, Catalog#ES002). Cleavage of ES002 can be measured using excitation and emission wavelength at 320 nm and 405 nm, respectively. The specific activity is >300 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)
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- Discover our top product CTSS 蛋白
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Cathepsin S (CTSS) (AA 1-331) protein (GST tag)
CTSS 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin S (CTSS) protein (His tag)CTSS 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Cathepsin S (CTSS) (Active) protein (His tag)CTSS 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Cathepsin S (CTSS) (AA 115-331) protein (His tag)Crystallography grade CTSS 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cathepsin S (CTSS) (AA 123-340) protein (His tag)Crystallography grade CTSS 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Cathepsin S (CTSS)
- 别名
- Cathepsin S/CTSS (CTSS 产品)
- 别名
- ctss-a Protein, MGC69026 Protein, ctss-b Protein, MGC80097 Protein, ctss.1 Protein, CTSS Protein, ctss Protein, cats Protein, cathepsin S Protein, cathepsin S L homeolog Protein, cathepsin S S homeolog Protein, Cathepsin S Protein, CTSS Protein, Ctss Protein, ctss.L Protein, ctss.S Protein, ctss Protein, cats Protein
- 背景
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Background: Cathepsin S (CTSS), one of the lysosomal proteinases, has many important physiological functions in the nervous system, especially in process of extracellular matrix degradation and endocellular antigen presentation. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. Cathepsin S is expressed in the lysosome of antigen presenting cells, primarily dendritic cells, B-cells and macrophages. Compared with other lysosomal cysteine proteases, cathepsin S has displayed some unique characteristics. Cathepsin S is most well known for its critical function in the proteolytic digestion of the invariant chain chaperone molecules, thus controlling antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules or to NK1.1+ T-cells via CD1 Molecules. Cathepsin S also appears to participate in direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells, or in cross-presentation by MHC class I molecules to CD8+ T-cells. In addition, although direct evidence is still lacking, in its secreted form cathepsin S is implicated in degradation of the extracellular matrix, which may contribute to the pathology of a number of diseases, including arthritis, atherosclerosis, neurological diseases and chronic obstructive pulmonary disease.
Synonym: Cathepsin S,CTSS,CTSS,MGC3886
- 分子量
- 37 kDa
- NCBI登录号
- NP_004070
- 途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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