Carboxypeptidase A2 Protein (His tag)
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- 抗原 See all Carboxypeptidase A2 (CPA2) 蛋白
- Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Carboxypeptidase A2 protein is labelled with His tag.
- 原理
- Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active)
- 序列
- Met 1-Tyr 417
- 产品特性
- A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-The specific activity is >4,000 pmoles/min/μg.
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- Discover our top product CPA2 蛋白
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Carboxypeptidase A2 (Pancreatic) (CPA2) protein (His tag)
CPA2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 1-417) protein (GST tag)CPA2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carboxypeptidase A2 (Pancreatic) (CPA2) (Active) protein (His tag)CPA2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
Carboxypeptidase A2 (Pancreatic) (CPA2) protein (His tag)CPA2 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 115-419) protein (His tag)Crystallography grade CPA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 113-417) protein (His tag)Crystallography grade CPA2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 25 mM Tris, 0.15 mM NaCl, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))
- 别名
- Carboxypeptidase A2/CPA2 (CPA2 产品)
- 别名
- CPA2 Protein, zgc:92530 Protein, carboxypeptidase A2 Protein, carboxypeptidase A2 (pancreatic) Protein, Carboxypeptidase A2 Protein, carboxypeptidase A2, pancreatic Protein, CPA2 Protein, cpa2 Protein, CpipJ_CPIJ001743 Protein, cbpa2 Protein, Cpa2 Protein
- 背景
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Background: Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Synonym: Carboxypeptidase A2, CPA2
- 分子量
- 46 kDa
- NCBI登录号
- NP_001860
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