Carboxypeptidase A2 Protein (AA 17-417) (His tag)
-
- 抗原 See all Carboxypeptidase A2 (CPA2) 蛋白
- Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-417
-
宿主
- 人
-
资源
- 人细胞
- 标记
- This Carboxypeptidase A2 protein is labelled with His tag.
- 原理
- Recombinant Human Carboxypeptidase A2/CPA2 (C-6His)
- 序列
- LETFVGDQVL EIVPSNEEQI KNLLQLEAQE HLQLDFWKSP TTPGETAHVR VPFVNVQAVK VFLGSQGIAY SIMIEDVQVL LDKENEEMLF NRRRERSGNF NFGAYHTLEE ISQEMDNLVA EHPGLVSKVN IGSSFENRPM NVLKFSTGGD KPAIWLDAGI HAREWVTQAT ALWTANKIVS DYGKDPSITS ILDALDIFLL PVTNPDGYVF SQTKNRMWRK TRSKVSGSLC VGVDPNRNWD AGFGGPGASS NPCSDSYHGP SANSEVEVKS IVDFIKSHGK VKAFITLHSY SQLLMFPYGY KCTKLDDFDE LSEVAQKAAQ SLRSLHGTKY KVGPICSVIY QASGGSIDWS YDYGIKYSFA FELRDTGRYG FLLPARQILP TAEETWLGLK AIMEHVRDHP YVDHHHHHH
- 产品特性
-
Recombinant Human Carboxypeptidase A2/CPA2 (C-6His)
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CPA2 蛋白
-
Carboxypeptidase A2 (Pancreatic) (CPA2) protein (His tag)
CPA2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 1-417) protein (GST tag)CPA2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carboxypeptidase A2 (Pancreatic) (CPA2) (Active) protein (His tag)CPA2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
Carboxypeptidase A2 (Pancreatic) (CPA2) protein (His tag)CPA2 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 115-419) protein (His tag)Crystallography grade CPA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carboxypeptidase A2 (Pancreatic) (CPA2) (AA 113-417) protein (His tag)Crystallography grade CPA2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150mm NaCl, pH 7.5.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))
- 别名
- Carboxypeptidase A2/CPA2 (CPA2 产品)
- 别名
- CPA2 Protein, zgc:92530 Protein, carboxypeptidase A2 Protein, carboxypeptidase A2 (pancreatic) Protein, Carboxypeptidase A2 Protein, carboxypeptidase A2, pancreatic Protein, CPA2 Protein, cpa2 Protein, CpipJ_CPIJ001743 Protein, cbpa2 Protein, Cpa2 Protein
- 背景
-
Recombinant Human Carboxypeptidase A2/CPA2 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser115-Tyr417) of Human CPA2 fused with a polyhistidine tag at the C-terminus.
Carboxypeptidase A2 (CPA) is a secreted pancreatic procarboxy-peptidase that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic AAs in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. - 分子量
- 45.91 kDa
- UniProt
- P48052
-
