CSK Protein (GST tag)
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- 抗原 See all CSK 蛋白
- CSK (C-Src tyrosine Kinase (CSK))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CSK protein is labelled with GST tag.
- 原理
- Recombinant Human CSK/C-Src kinase Protein (GST Tag)(Active)
- 序列
- Met 1-Leu 450
- 产品特性
- A DNA sequence encoding the human CSK (NP_004374.1) (Met 1-Leu 450) was fused with the GST tag at the N-terminus.
- 纯度
- > 92 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 127 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
- Top Product
- Discover our top product CSK 蛋白
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C-Src tyrosine Kinase (CSK) (AA 1-450) protein (GST tag)
CSK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
C-Src tyrosine Kinase (CSK) (AA 1-450) (Active) protein (His-GST)CSK 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
C-Src tyrosine Kinase (CSK) (AA 1-450) protein (His tag)CSK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
C-Src tyrosine Kinase (CSK) (AA 1-100) protein (GST tag)CSK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
C-Src tyrosine Kinase (CSK) proteinCSK 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
C-Src tyrosine Kinase (CSK) (Active) protein (GST tag,His tag)CSK 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
C-Src tyrosine Kinase (CSK) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)CSK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- CSK (C-Src tyrosine Kinase (CSK))
- 别名
- CSK/C-Src kinase (CSK 产品)
- 别名
- AW212630 Protein, C-terminal Src kinase Protein, c-src tyrosine kinase Protein, CSK, non-receptor tyrosine kinase Protein, CSK Protein, Csk Protein
- 背景
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Background: The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development. References
Synonym: MGC117393,CSK
- 分子量
- 77 kDa
- NCBI登录号
- NP_004374
- 途径
- TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
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