BCAM Protein (His tag)
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- 抗原 See all BCAM 蛋白
- BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 大鼠
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资源
- HEK-293 Cells
- 标记
- This BCAM protein is labelled with His tag.
- 原理
- Recombinant Rat BCAM Protein (His Tag)(Active)
- 序列
- Met1-Ala543
- 产品特性
- A DNA sequence encoding the rat BCAM (Q9ESS6) (Met1-Ala543) was expressed, fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.When cells are added to coated plates(10 μg/mL,100μL/well), approximately 20-50% will adhere after 1 hour at 37°C.
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- Discover our top product BCAM 蛋白
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Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-547) protein (His tag)
BCAM 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 26-541) protein (His tag)BCAM 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-548) protein (His tag)BCAM 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-131) protein (GST tag)BCAM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-547) protein (His tag)Crystallography grade BCAM 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 26-541) protein (His tag)Crystallography grade BCAM 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-628) protein (rho-1D4 tag)Crystallography grade BCAM 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 26-622) protein (rho-1D4 tag)Crystallography grade BCAM 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))
- 别名
- BCAM (BCAM 产品)
- 别名
- cd239 Protein, msk19 Protein, MGC81012 Protein, LU Protein, prr2 Protein, zgc:154034 Protein, 1200005K12Rik Protein, B-CAM Protein, Gplu Protein, Lu Protein, AU Protein, CD239 Protein, MSK19 Protein, basal cell adhesion molecule (Lutheran blood group) Protein, basal cell adhesion molecule (Lutheran blood group) L homeolog Protein, basal cell adhesion molecule Protein, bcam Protein, bcam.L Protein, BCAM Protein, Bcam Protein
- 背景
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Background: The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.
Synonym: BCAM
- 分子量
- 58.2 kDa
- UniProt
- Q9ESS6
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