BIRC2 Protein (AVI tag)
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- 抗原 See all BIRC2 蛋白
- BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This BIRC2 protein is labelled with AVI tag.
- 原理
- Recombinant Human cIAP1/HIAP2 Protein (AVI Tag)(Active)
- 序列
- Glu 144-Leu 356
- 产品特性
- A DNA sequence encoding the BIR2 & BIR3 domains (Glu 144-Leu 356) of human cIAP1 (NP_001157.1) was expressed, fused with the AVI tag at the C-terminus, and two additional amino acids (Gly & Pro) at the N-terminus.
- 纯度
- > 92 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. The IC50 for this effect is typically 25-750 nM.
- Top Product
- Discover our top product BIRC2 蛋白
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Baculoviral IAP Repeat Containing 2 (BIRC2) (AA 1-618) protein (GST tag)
BIRC2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Baculoviral IAP Repeat Containing 2 (BIRC2) (AA 1-618) protein (Strep Tag)Crystallography grade BIRC2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Baculoviral IAP Repeat Containing 2 (BIRC2) (AA 1-612) protein (Strep Tag)Crystallography grade BIRC2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Baculoviral IAP Repeat Containing 2 (BIRC2) protein (Myc-DYKDDDDK Tag)BIRC2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 10 mM Tris, 5 % glycerol, 0.5 mM EDTA, 5 mM DTT, pH 7.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))
- 别名
- cIAP1/HIAP2 (BIRC2 产品)
- 别名
- API1 Protein, HIAP2 Protein, Hiap-2 Protein, MIHB Protein, RNF48 Protein, c-IAP1 Protein, cIAP1 Protein, Api2 Protein, rIAP1 Protein, IAP1 Protein, ITA Protein, AW146227 Protein, Api1 Protein, Birc3 Protein, HIAP1 Protein, IAP2 Protein, MIAP1 Protein, MIAP2 Protein, MIHC Protein, cIAP2 Protein, mcIAP1 Protein, ciap-1 Protein, birc3 Protein, api1 Protein, birc2 Protein, c-iap1 Protein, ciap1 Protein, hiap-2 Protein, hiap2 Protein, mihb Protein, rnf48 Protein, baculoviral IAP repeat containing 2 Protein, baculoviral IAP repeat-containing 2 Protein, baculoviral IAP repeat containing 2 L homeolog Protein, BIRC2 Protein, Birc2 Protein, birc2 Protein, birc2.L Protein
- 背景
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Background: The cellular inhibitor of apoptosis protein-1 (cIAP1) is a member of the Inhibitor of Apoptosis family proteinsare (IAP) whose members are characterized by a novel domain of about 70 amino acids termed baculoviral IAP repeats (BIRs). The BIR domains of cIAP1 and cIAP2 bind to caspases, the key effector proteases of apoptosis. The IAP protein family which can enhance cell survival are crucial regulators of programmed cell death. Both cIAP1 and cIAP2 are the E3 ubiquitin protein isopeptide ligases for Smac, taking part in promoting cancer survival through functioning as E3 ubiqitin ligases. Removal of cIAP1 by genetic deletion may result in NF-κB signaling activation that induces TNFα production and in killing sensitive tumor cells through enhanced TNF-R1 death-receptor signaling and caspase 8 activation. The substrate-dependent E3 activity of cIAPs is mediated by their RING domains and is dependent on the specific interactions between cIAPs and Smac. cIAP1 and cIAP2 are also reported to be regulators of NF-kB activation upon TNFαtreatment.
Synonym: API1;c-IAP1;cIAP1;Hiap-2;HIAP2;MIHB;RNF48
- 分子量
- 26.5 kDa
- NCBI登录号
- NP_001157
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades
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