Aminoacylase 1 Protein (ACY1) (His tag)
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- 抗原 See all Aminoacylase 1 (ACY1) 蛋白
- Aminoacylase 1 (ACY1)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Aminoacylase 1 protein is labelled with His tag.
- 原理
- Recombinant Mouse ACY1/Aminoacylase-1 Protein (His Tag)(Active)
- 序列
- Met 1-Ser 408
- 产品特性
- A DNA sequence encoding the mouse ACY1 (Q99JW2) (Met 1-Ser 408) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 95 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave N-acetyl-L-Methione (Ac-Met). The specific activity is >4,000 pmoles/min/μg.
- Top Product
- Discover our top product ACY1 蛋白
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Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)
ACY1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)ACY1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (GST tag)ACY1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Aminoacylase 1 (ACY1) protein (DYKDDDDK Tag)ACY1 宿主: 人 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Aminoacylase 1 (ACY1) protein (Myc-DYKDDDDK Tag)ACY1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Aminoacylase 1 (ACY1)
- 别名
- ACY1/Aminoacylase-1 (ACY1 产品)
- 别名
- si:zfos-226g1.1 Protein, zgc:55605 Protein, zgc:76955 Protein, acy1 Protein, acy1d Protein, acylase Protein, ACY1 Protein, ACY-1 Protein, ACY1D Protein, 1110014J22Rik Protein, Acy-1 Protein, Acy1a Protein, Acy1b Protein, aminoacylase 1 Protein, aminoacylase 1, gene 1 S homeolog Protein, aminoacylase 1, gene 1 Protein, ACY1 Protein, acy1 Protein, acy1.1.S Protein, acy1.1 Protein, Acy1 Protein
- 背景
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Background: Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Synonym: 1110014J22Rik,Acy-1
- 分子量
- 45 kDa
- UniProt
- Q99JW2
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