Aminoacylase 1 Protein (ACY1) (His tag)
Our Local Distributor
北京 101111
Quick Overview for Aminoacylase 1 Protein (ACY1) (His tag) (ABIN7317832)
抗原
See all Aminoacylase 1 (ACY1) 蛋白蛋白类型
宿主
资源
纯度
-
-
标记
- This Aminoacylase 1 protein is labelled with His tag.
-
原理
- Recombinant Human ACY1/Aminoacylase-1 Protein (His Tag)
-
序列
- Met 1-Ser 408
-
产品特性
- A DNA sequence encoding the full length of human ACY1 (NP_000657.1) (Met 1-Ser 408) was expressed with a polyhistidine tag at the C-terminus.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Not validated for activity
-
-
Aminoacylase 1 (ACY1) (AA 1-408) protein (His tag)
ACY1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Aminoacylase 1 (ACY1) (AA 1-408) protein (His-IF2DI Tag)ACY1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Aminoacylase 1 (ACY1) (AA 1-408) protein (His-IF2DI Tag)ACY1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Aminoacylase 1 (ACY1) (Active) protein (His tag)ACY1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Aminoacylase 1 (ACY1) (AA 1-408) protein (GST tag)ACY1 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Aminoacylase 1 (ACY1) protein (Myc-DYKDDDDK Tag)ACY1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- Aminoacylase 1 (ACY1)
-
别名
- ACY1/Aminoacylase-1
-
背景
- ACY-1,ACY1D,HEL-S-5,Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
-
分子量
-
Calculated MW: 47.3 kDa
Observed MW: 44 kDa
-
基因ID
- 95
-
NCBI登录号
- NP_000657
-
UniProt
- Q03154
抗原
-
