PFKFB3 Protein (GST tag,His tag)
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- 抗原 See all PFKFB3 蛋白
- PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This PFKFB3 protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human PFK2/PFKFB3 Protein (His & GST Tag)
- 序列
- Met 1-His 520
- 产品特性
- A DNA sequence encoding the human PFKFB3 isoform 1 (Q16875-1) (Met 1-His 520) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 85 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product PFKFB3 蛋白
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6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) (AA 1-520) protein (GST tag)
PFKFB3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) (AA 412-520) protein (GST tag)PFKFB3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3) protein (His tag)PFKFB3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.0, 10 % glycerol, 0.3 mM DTT
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))
- 别名
- PFK2/PFKFB3 (PFKFB3 产品)
- 别名
- IPFK2 Protein, PFK2 Protein, E330010H22Rik Protein, iPFK-2 Protein, uPFK-2 Protein, PFKFB3 Protein, zgc:73257 Protein, wu:fc31e01 Protein, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 Protein, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 S homeolog Protein, PFKFB3 Protein, Pfkfb3 Protein, pfkfb3.S Protein, pfkfb3 Protein, PTRG_09894 Protein, Tsp_06710 Protein
- 背景
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Background: Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2,6-bisphosphate (Fru-2,6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFK-2 / FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2,6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions.
Synonym: IPFK2,PFK2
- 分子量
- 87.4 kDa
- 途径
- AMPK Signaling, Regulation of Carbohydrate Metabolic Process
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