TPI1 Protein (full length) (rho-1D4 tag)
-
- 抗原 See all TPI1 蛋白
- TPI1 (Triosephosphate Isomerase 1 (TPI1))
- 蛋白类型
- Recombinant
- 产品特性
- full length
-
宿主
- CHO cells
-
资源
- Insect Cells
- 标记
- This TPI1 protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), Functional Studies (Func)
- 序列
-
MVPSRKFFVG GNWKMTGKKK CLGELICTLN AAKVQADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV NNGVFTGEIS PGMIKDLGAE WVVLGHSERR HIFGESDELI GQKVVHALAE GLGVIACIGE KLDDRHHREG CFRANQGHCR
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Triosephosphate isomerase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product TPI1 蛋白
-
Triosephosphate Isomerase 1 (TPI1) (AA 1-249) protein (GST tag)
TPI1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Triosephosphate Isomerase 1 (TPI1) (AA 1-249) protein (His tag)TPI1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Triosephosphate Isomerase 1 (TPI1) (AA 1-286) protein (His tag)Crystallography grade TPI1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Triosephosphate Isomerase 1 (TPI1) (AA 1-299) protein (His tag)Crystallography grade TPI1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Triosephosphate Isomerase 1 (TPI1) (AA 1-249) (Active) protein (His tag)TPI1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active
Triosephosphate Isomerase 1 (TPI1) protein (Myc-DYKDDDDK Tag)TPI1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- TPI1 (Triosephosphate Isomerase 1 (TPI1))
- 别名
- Triosephosphate isomerase (TPI1 产品)
- 别名
- TIM Protein, TPI Protein, TIM1 Protein, hTIM Protein, tpi Protein, tpi1 Protein, TPI1 Protein, AI255506 Protein, Tpi Protein, Tpi-1 Protein, GB17473 Protein, Tim Protein, CG2171 Protein, Dmel\\CG2171 Protein, ND14 Protein, TPIS Protein, sgk Protein, wstd Protein, DrTPI-A Protein, DrTPI-B Protein, TIM-B Protein, cb179 Protein, wu:fb51c02 Protein, triosephosphate isomerase 1 Protein, timeless circadian regulator Protein, triosephosphate isomerase 1 S homeolog Protein, triosephosphate isomerase Protein, triose-phosphate isomerase Protein, Triose-phosphate isomerase Protein, triose-phosphate isomerase, putative Protein, timeless circadian clock Protein, Triose phosphate isomerase Protein, Triosephosphate isomerase Protein, triosephosphate isomerase 1a Protein, triosephosphate isomerase 1b Protein, TPI1 Protein, TIMELESS Protein, tpi1 Protein, Tpi1 Protein, tpi1.S Protein, Tpi Protein, tpiA Protein, CNE05000 Protein, Fnod_0738 Protein, Tlet_0996 Protein, SARE_RS16215 Protein, PKH_126640 Protein, Timeless Protein, tpi-1 Protein, tpi1a Protein, tpi1b Protein
- 背景
- It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids. {ECO:0000256|ARBA:ARBA00004104}., Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis. {ECO:0000256|ARBA:ARBA00002041}.
- UniProt
- G3HNV4
- 途径
- Cell RedoxHomeostasis
-
