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TPI1 Protein (AA 1-249) (His tag)

Name: TPI1 Protein (AA 1-249) (His tag)
SKU: ABIN6387938
Availability: OutOfStock

TPI1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS Active

SDS-PAGE (SDS) image for Triosephosphate Isomerase 1 (TPI1) (AA 1-249) (Active) protein (His tag) (ABIN6387938) (TPI1 Protein (AA 1-249) (His tag))

SDS

1 image

产品编号 ABIN6387938

发货至: 中国

Datasheet as PDF

抗原 See all TPI1 蛋白

TPI1 (Triosephosphate Isomerase 1 (TPI1))

蛋白类型

Recombinant

生物活性

Active

产品特性

AA 1-249
宿主
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资源
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大肠杆菌（E. Coli）

标记

This TPI1 protein is labelled with His tag.

应用范围

SDS-PAGE (SDS)

序列

MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

纯度

> 95 % by SDS - PAGE

内毒素水平

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment

Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.

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Triosephosphate Isomerase 1 (TPI1) (AA 1-249) protein (His tag)
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Triosephosphate Isomerase 1 (TPI1) (AA 1-299) protein (His tag)
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Triosephosphate Isomerase 1 (TPI1) protein (His tag)
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Triosephosphate Isomerase 1 (TPI1) (AA 1-286) protein (His tag)
TPI1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, Imm, PC

Triosephosphate Isomerase 1 (TPI1) (Active) protein (His tag)
TPI1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant SDS Active

Triosephosphate Isomerase 1 (TPI1) protein (His tag)
TPI1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by reducing SDS-PAGE.

Triosephosphate Isomerase 1 (TPI1) (full length) protein (rho-1D4 tag)
Crystallography grade TPI1 宿主: CHO cells 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys, Func

Triosephosphate Isomerase 1 (TPI1) protein (Myc-DYKDDDDK Tag)
TPI1 宿主: 人宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD

应用备注

Optimal working dilution should be determined by the investigator.

说明

Bioactivity Validated

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol, 1  mM DTT.

储存条件

4 °C,-20 °C,-80 °C

储存方法

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
抗原

TPI1 (Triosephosphate Isomerase 1 (TPI1))

别名

TPI1 (TPI1 产品)

别名

TIM Protein, TPI Protein, TIM1 Protein, hTIM Protein, tpi Protein, tpi1 Protein, TPI1 Protein, AI255506 Protein, Tpi Protein, Tpi-1 Protein, GB17473 Protein, Tim Protein, CG2171 Protein, Dmel\\CG2171 Protein, ND14 Protein, TPIS Protein, sgk Protein, wstd Protein, DrTPI-A Protein, DrTPI-B Protein, TIM-B Protein, cb179 Protein, wu:fb51c02 Protein, triosephosphate isomerase 1 Protein, timeless circadian regulator Protein, triosephosphate isomerase 1 S homeolog Protein, triosephosphate isomerase Protein, triose-phosphate isomerase Protein, Triose-phosphate isomerase Protein, triose-phosphate isomerase, putative Protein, timeless circadian clock Protein, Triose phosphate isomerase Protein, Triosephosphate isomerase Protein, triosephosphate isomerase 1a Protein, triosephosphate isomerase 1b Protein, TPI1 Protein, TIMELESS Protein, tpi1 Protein, Tpi1 Protein, tpi1.S Protein, Tpi Protein, tpiA Protein, CNE05000 Protein, Fnod_0738 Protein, Tlet_0996 Protein, SARE_RS16215 Protein, PKH_126640 Protein, Timeless Protein, tpi-1 Protein, tpi1a Protein, tpi1b Protein

背景

TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

分子量

28.8kDa (269aa) confirmed by MALDI-TOF

NCBI登录号

NP_000356

UniProt

P60174

途径

Cell RedoxHomeostasis