GAPDH Protein (full length) (rho-1D4 tag)
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- 抗原 See all GAPDH 蛋白
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This GAPDH protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MVKVGVNGFG RIGRLVTRAA FTSGKVEVVA INDPFIDLNY MVYMFQYDST HGKFKGTVKA ENGKLVINGK AITIFQERDP ANIKWGDAGA EYVVESTGVF TTMEKAGAHL KGGAKRVIIS APSADAPMFV MGVNQDKYDN SLKIVSNASC TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA AQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTCRLEKP AKYEDIKKVV KQASEGPLKG ILGYTEDQVV SCDFNSDSHS STFDAGAGIA LNDNFVKLIS WYDNEFGYSN RVVDLMAYMA SKE
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product GAPDH 蛋白
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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-333) protein (His tag)
GAPDH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-335) protein (His tag)GAPDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) protein (GST tag)GAPDH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) protein (GST tag)GAPDH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-333) protein (His tag)Crystallography grade GAPDH 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Abstract
- GAPDH 产品
- 别名
- G3PD Protein, GAPD Protein, Gapd Protein, BEST:GH12586 Protein, CG12055 Protein, Dmel\\CG12055 Protein, GA3PDH Protein, GADPH Protein, GAP Protein, GAPDH Protein, GAPDH I Protein, GAPDH-1 Protein, GAPDH1 Protein, GAPDHI Protein, Gapdh Protein, Gapdh-1 Protein, Gapdh43E Protein, gadph Protein, gapdh Protein, gapdh-1 Protein, gh12586 Protein, cb609 Protein, gapd Protein, mg:bb02e05 Protein, wu:fb33a10 Protein, wu:ft80f05 Protein, KNC-NDS6 Protein, g3pd Protein, G3PDH Protein, glyceraldehyde-3-phosphate dehydrogenase Protein, Glyceraldehyde 3 phosphate dehydrogenase 1 Protein, glyceraldehyde-3-phosphate dehydrogenase S homeolog Protein, glyceraldehyde-3-phosphate dehydrogenase, type I Protein, olfactory receptor 8K3 Protein, GAPDH Protein, Gapdh Protein, Gapdh1 Protein, gapdh Protein, gapdh.S Protein, gapDH Protein, LOC100404960 Protein, LOC614985 Protein
- 背景
- Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (By similarity). Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). {ECO:0000250|UniProtKB:P04406, ECO:0000250|UniProtKB:P04797}.
- UniProt
- P17244
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