HSP90AB1 Protein (full length) (rho-1D4 tag)
-
- 抗原 See all HSP90AB1 蛋白
- HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
- 蛋白类型
- Recombinant
- 产品特性
- full length
-
宿主
- CHO cells
-
资源
- Insect Cells
- 标记
- This HSP90AB1 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)
- 序列
-
MYYWVFQHFS VEGQLEFRAL LFIPRRAPFD LFENKKKKNN IKLYVRRVFI MDSCDELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNIVKKC LELFSELAED KENYKKFYEA FSKNLKLGIH EDSTNRRRLS ELLRYHTSQS GDEMTSLSEY VSRMKETQKS IYYITGESKE QVANSAFVER VPKRGFEAVY MTEPIDEYCV QQLKEFDGKS LVSVTKEGLE LPEDEEEKKK MEESKAKFEN LCKLMKEILD KKVEKVTISN RLVSSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMMA KKHLEINPDH PIVETLRQKA EADKNDKAVK DLVVLLFETA LLSSGFSLED PQTHSNRIYR MIKLGLGIDE DEVTAEEPSA AVPDEIPPLE GDEDASRMEE VD
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Heat shock protein HSP 90-beta Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product HSP90AB1 蛋白
-
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (full length) (Active) protein
HSP90AB1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant >90% WB, ELISA, SDS, Func, Co-IP, SPR Active
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (AA 1-724) protein (GST tag)HSP90AB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (AA 2-724) protein (His tag)Crystallography grade HSP90AB1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (AA 2-724) protein (His tag)Crystallography grade HSP90AB1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) protein (Myc-DYKDDDDK Tag)HSP90AB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
- 别名
- Heat shock protein HSP 90-beta (HSP90AB1 产品)
- 别名
- D6S182 Protein, HSP84 Protein, HSP90B Protein, HSPC2 Protein, HSPCB Protein, GRP94 Protein, TRA1 Protein, hsp90b Protein, 90kDa Protein, AL022974 Protein, C81438 Protein, Hsp84 Protein, Hsp84-1 Protein, Hsp90 Protein, Hspcb Protein, Hsp70 Protein, Hsp70-1 Protein, Hsp70.1 Protein, hsp68 Protein, HSP90-BETA Protein, hsp90beta Protein, wu:fa29f01 Protein, wu:fa91e11 Protein, wu:fd59e11 Protein, wu:gcd22h07 Protein, HSP90 Protein, heat shock protein 90 alpha family class B member 1 Protein, heat shock protein 90 beta family member 1 Protein, Heat Shock Protein 90, endoplasmic reticulum Protein, heat shock protein 90B Protein, heat shock protein 90 alpha (cytosolic), class B member 1 Protein, heat shock protein 1B Protein, heat shock protein 90kDa alpha family class B member 1 S homeolog Protein, heat shock protein 90, alpha (cytosolic), class B member 1 Protein, HSP90AB1 Protein, HSP90B1 Protein, HSP90B Protein, hsp90ab1 Protein, Hsp90ab1 Protein, Hspa1b Protein, hsp90ab1.S Protein
- UniProt
- G3HC84
- 途径
- Regulation of Cell Size
-
