HSP90AB1 Protein (AA 2-724) (His tag)
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- 抗原 See all HSP90AB1 蛋白
- HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-724
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This HSP90AB1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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PEEVHHGEEE VETFAFQAEI AQLMSLIINT FYSNKEIFLR ELISNASDAL DKIRYESLTD PSKLDSGKEL KIDIIPNPQE RTLTLVDTGI GMTKADLINN LGTIAKSGTK AFMEALQAGA DISMIGQFGV GFYSAYLVAE KVVVITKHND DEQYAWESSA GGSFTVRADH GEPIGRGTKV ILHLKEDQTE YLEERRVKEV VKKHSQFIGY PITLYLEKER EKEISDDEAE EEKGEKEEED KEDEEKPKIE DVGSDEEDDS GKDKKKKTKK IKEKYIDQEE LNKTKPIWTR NPDDITQEEY GEFYKSLTND WEDHLAVKHF SVEGQLEFRA LLFIPRRAPF DLFENKKKKN NIKLYVRRVF IMDSCDELIP EYLNFIRGVV DSEDLPLNIS REMLQQSKIL KVIRKNIVKK CLELFSELAE DKENYKKFYE AFSKNLKLGI HEDSTNRRRL SELLRYHTSQ SGDEMTSLSE YVSRMKETQK SIYYITGESK EQVANSAFVE RVRKRGFEVV YMTEPIDEYC VQQLKEFDGK SLVSVTKEGL ELPEDEEEKK KMEESKAKFE NLCKLMKEIL DKKVEKVTIS NRLVSSPCCI VTSTYGWTAN MERIMKAQAL RDNSTMGYMM AKKHLEINPD HPIVETLRQK AEADKNDKAV KDLVVLLFET ALLSSGFSLE DPQTHSNRIY RMIKLGLGID EDEVTAEEPS AAVPDEIPPL EGDEDASRME EVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Hsp90ab1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HSP90AB1 蛋白
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Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (full length) (Active) protein
HSP90AB1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant >90% WB, ELISA, SDS, Func, Co-IP, SPR Active
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (AA 1-724) protein (GST tag)HSP90AB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) (AA 2-724) protein (His tag)Crystallography grade HSP90AB1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1) protein (Myc-DYKDDDDK Tag)HSP90AB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
- 别名
- Hsp90ab1 (HSP90AB1 产品)
- 别名
- D6S182 Protein, HSP84 Protein, HSP90B Protein, HSPC2 Protein, HSPCB Protein, GRP94 Protein, TRA1 Protein, hsp90b Protein, 90kDa Protein, AL022974 Protein, C81438 Protein, Hsp84 Protein, Hsp84-1 Protein, Hsp90 Protein, Hspcb Protein, Hsp70 Protein, Hsp70-1 Protein, Hsp70.1 Protein, hsp68 Protein, HSP90-BETA Protein, hsp90beta Protein, wu:fa29f01 Protein, wu:fa91e11 Protein, wu:fd59e11 Protein, wu:gcd22h07 Protein, HSP90 Protein, heat shock protein 90 alpha family class B member 1 Protein, heat shock protein 90 beta family member 1 Protein, Heat Shock Protein 90, endoplasmic reticulum Protein, heat shock protein 90B Protein, heat shock protein 90 alpha (cytosolic), class B member 1 Protein, heat shock protein 1B Protein, heat shock protein 90kDa alpha family class B member 1 S homeolog Protein, heat shock protein 90, alpha (cytosolic), class B member 1 Protein, HSP90AB1 Protein, HSP90B1 Protein, HSP90B Protein, hsp90ab1 Protein, Hsp90ab1 Protein, Hspa1b Protein, hsp90ab1.S Protein
- 背景
- Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). {ECO:0000250}.
- 分子量
- 84.1 kDa Including tag.
- UniProt
- P11499
- 途径
- Regulation of Cell Size
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