HHLA2 Protein (His-Avi Tag,Biotin)
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- 抗原 See all HHLA2 蛋白
- HHLA2 (HERV-H LTR-Associating 2 (HHLA2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This HHLA2 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human B7-H7 / HHLA2 Protein, His,Avitag™ (MALS verified)
- 序列
- Ile 23 - Asn 344
- 产品特性
- Biotinylated Human B7-H7, His,Avitag™ is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
- Top Product
- Discover our top product HHLA2 蛋白
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HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) protein (His tag)
HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) protein (Fc Tag)HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HERV-H LTR-Associating 2 (HHLA2) (AA 1-414) protein (GST tag)HHLA2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) (Active) protein (Fc Tag)HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) (Active) protein (Fc Tag,AVI tag,Biotin)HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 40.7 kDa. The protein migrates as 50-66 kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- HHLA2 (HERV-H LTR-Associating 2 (HHLA2))
- 别名
- B7-H7 / HHLA2 (HHLA2 产品)
- 别名
- B7H7 Protein, HERV-H LTR-associating 2 Protein, HHLA2 Protein, hhla2 Protein
- 背景
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Synonyms: B7-H7,HHLA2,B7 Homolog 7,
Description: B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members. - 分子量
- 40.7 kDa
- NCBI登录号
- NP_009003
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