Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6xHis,Avi tag at the C-terminus.
纯度
> 95 % by SDS-PAGE.
过滤
0.22 μm filtered
内毒素水平
< 1.0 EU/μg of the protein by LAL method.
Biological Activity Comment
Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 at 2 μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD with a linear range of 0.03-4.9 ng/mL.
ACE2
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
缓冲液
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
4 °C,-20 °C,-80 °C
储存方法
Store the lyophilized protein at -20°C to -80 °C for long term.After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.