TNF alpha 蛋白
(1 reference)
-
- 抗原 See all TNF alpha 蛋白
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 蛋白类型
- Recombinant
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-321
- Supplier
- Rockland
- 原理
- TNF-α Mouse Recombinant Protein
- 纯化方法
- Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE.
- 纯度
- 98,00%
- 内毒素水平
- Measured by LAL is 0.04 EU/μg protein (with 50% confidence range).
- Biological Activity Comment
- Recombinant Mouse TNFα is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is 13-20 pg/mL.
- Top Product
- Discover our top product TNF alpha 蛋白
-
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha 宿主: 兔 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag)TNF alpha 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)TNF alpha 宿主: 犬 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
-
- 应用备注
-
Other: User Optimized
Application_Note: TNFα protein has been tested by SDDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.
- 说明
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
- 浓度
- 0.1 mg/mL
- 缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
-
-
: "Role of TRPM2 in H(2)O(2)-induced cell apoptosis in endothelial cells." in: PloS one, Vol. 7, Issue 8, pp. e43186, (2013) (PubMed).
-
: "Role of TRPM2 in H(2)O(2)-induced cell apoptosis in endothelial cells." in: PloS one, Vol. 7, Issue 8, pp. e43186, (2013) (PubMed).
-
- 抗原
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- Tnf (TNF alpha 产品)
- 背景
-
Synonyms: Tumor necrosis factor, TNF-alpha, TNF-a, TNFα, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin
Background: Tumor Necrosis Factor-alpha (TNF-a) is pleiotrophic cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following stimulation by bacterial LPS . TNF-a activity is mediated via interactions with TNFR1 &TNFR2 receptors. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 & GM-CSF. Recombinant mouse TNF-a produced in E.Coli is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 17,300 daltons.
- UniProt
- P06804
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
-
