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TNF alpha 蛋白

Name: TNF alpha 蛋白
SKU: ABIN6700748
Availability: OutOfStock

This Recombinant TNF alpha protein is expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

TNF-a Mouse Cytokine - SDS-PAGE. (TNF alpha 蛋白)

SDS

1 image

(1 reference)

产品编号 ABIN6700748

Supplier Product No.: 010-001-321

发货至: 中国

Datasheet as PDF

Quick Overview for TNF alpha 蛋白 (ABIN6700748)

抗原

See all TNF alpha 蛋白

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

蛋白类型

Recombinant

宿主

小鼠

资源

大肠杆菌（E. Coli）

应用范围

SDS-PAGE (SDS)

纯度

98,00%

Discover our top product TNF alpha 蛋白

Supplier Product No.

010-001-321

Supplier

Rockland

原理

TNF-α Mouse Recombinant Protein

纯化方法

Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE.

内毒素水平

Measured by LAL is 0.04 EU/μg protein (with 50% confidence range).

Biological Activity Comment

Recombinant Mouse TNFα is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is 13-20 pg/mL.
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Product

表达系统

Conjugate

Origin

价格从

TNF alpha Protein (AA 1-235) (His tag)

表达系统 HEK-293 Cells

Conjugate His tag

Origin Mouse

价格从 136,947.45 ¥

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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)
custom-made TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)
custom-made TNF alpha 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein
HPLC verified TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 兔宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active

应用备注

Application Note: TNFα protein has been tested by SDDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)

浓度

0.1 mg/mL

缓冲液

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None

储存液

Without preservative

储存条件

-20 °C

储存方法

Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

有效期

6 months
Sun, Yau, Wong, Li, Huang, Yao: "Role of TRPM2 in H(2)O(2)-induced cell apoptosis in endothelial cells." in: PloS one, Vol. 7, Issue 8, pp. e43186, (2013) (PubMed).
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

Tnf

背景

Synonyms: Tumor necrosis factor, TNF-alpha, TNF-a, TNFα, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin
Background: Tumor Necrosis Factor-alpha (TNF-a) is pleiotrophic cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following stimulation by bacterial LPS . TNF-a activity is mediated via interactions with TNFR1 &TNFR2 receptors. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 & GM-CSF. Recombinant mouse TNF-a produced in E.Coli is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 17,300 daltons.

UniProt

P06804

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome