C-JUN Protein (GST tag)
Quick Overview for C-JUN Protein (GST tag) (ABIN6700182)
抗原
See all C-JUN (JUN) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This C-JUN protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r71s
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Supplier
- Rockland
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原理
- c-JUN recombinant protein-GST fusion protein
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纯化方法
- Recombinant human c-JUN (1-79) expressed in E. coli cells using an N-Terminal GST Protein. The purity was determined to be >50% by densitometry.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 172,528.32 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Jun Proto-Oncogene (JUN) (AA 1-331) protein (His tag)
custom-made JUN 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Jun Proto-Oncogene (JUN) (AA 1-334) protein (His tag)custom-made JUN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Jun Proto-Oncogene (JUN) (AA 1-241) protein (His tag)JUN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Jun Proto-Oncogene (JUN) (AA 75-274) protein (His tag)JUN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Jun Proto-Oncogene (JUN) (AA 1-331) protein (GST tag)JUN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Application Note: c-JUN Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. c-JUN Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 35 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: Kinase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- c-JUN Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
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别名
- JUN
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背景
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Synonyms: AP1, Transcription factor AP-1, Activator protein 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
Background: c-Jun is a proto-oncogene that forms a complex with c-Fos which regulates transcription from promoters containing AP-1 activation elements (1). c-Jun has specific DNA binding activity and following in vitro translation, c-Jun binds as a homodimer to the AP-1 DNA site. The transactivating function of c-Jun is acutely regulated by a wide variety of cellular signals via modulation of phosphorylation of two serines (63 and 73). Jun N-terminal domain kinases (JNKs), are responsible for mediating S63/73 phosphorylation on c-Jun in response to a variety of cellular stimuli including TNF-a, heat stress and u.v. light (2). c-JUN Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, and NfkB Pathway research.
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NCBI登录号
- NM_002228
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途径
- MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
抗原
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