Quick Overview for IL-6 蛋白 (ABIN6700155)
抗原
See all IL-6 (IL6) 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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Supplier Product No.
- 009-f01-310-0005
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Supplier
- Rockland
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原理
- Human Interleukin-6 Recombinant Protein (Animal Free)
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纯化方法
- Interleukin-6 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent stimulation of mouse 7TD1 cells or B9 cell proliferation and is typically 250 pg/mL or 25 pg/mL or 4 x 10^6 units/mg or 4 x 10^7 units/mg.
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Interleukin 6 (IL6) (AA 30-212) protein (mFc-His Tag)
IL6 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
Interleukin 6 (IL6) (AA 30-212) proteinIL6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 6 (IL6) (AA 28-212) protein (His tag)IL6 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 6 (IL6) (AA 29-212) (Active) proteinIL6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 6 (IL6) (AA 25-211) (Active) protein (His tag)IL6 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 6 (IL6) (AA 30-212) (Active) proteinIL6 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 6 (IL6) (AA 30-212) (Active) protein (His tag)IL6 宿主: 人 宿主: HEK-293 Cells > 97 % AcA, CC Active
Interleukin 6 (IL6) (AA 30-212) (Active) proteinIL6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
Interleukin 6 (IL6) (AA 25-211) (Active) protein (His tag)IL6 宿主: 大鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
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应用备注
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Application Note: Interleukin-6 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-6 (IL6) (Interleukin 6 (IL6))
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别名
- IL6
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背景
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Synonyms: Interferon beta-2 (IFN-β2), B-cell Differentiation Factor (BSF-2), CTL differentiation factor (CDF), HSF, Hybridoma growthfactor, MGI-2
Background: Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells. Recombinant human IL-6 is a non-glycosylated protein, containing 184 amino acids, with a molecular weight of 21 kDa.
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UniProt
- P05231
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途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
抗原
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