ADK Protein (AA 1-361) (His tag)
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- 抗原 See all ADK 蛋白
- ADK (Adenosine Kinase (ADK))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-361
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ADK protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.
- Top Product
- Discover our top product ADK 蛋白
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Adenosine Kinase (ADK) (AA 22-362) protein (His tag)
ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Adenosine Kinase (ADK) (AA 1-345) protein (GST tag)ADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine Kinase (ADK) (AA 236-345) protein (GST tag)ADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine Kinase (ADK) protein (GST tag,His tag)ADK 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Adenosine Kinase (ADK) (AA 1-361) protein (Strep Tag)Crystallography grade ADK 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Adenosine Kinase (ADK) (AA 2-362) protein (His tag)Crystallography grade ADK 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 20 % glycerol, 50 mM NaCl, 1 mM EDTA
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- ADK (Adenosine Kinase (ADK))
- 别名
- Adk (ADK 产品)
- 别名
- AK Protein, ADK Protein, DDBDRAFT_0186795 Protein, DDBDRAFT_0230174 Protein, DDB_0186795 Protein, DDB_0230174 Protein, ATPADK1 Protein, F3G5.4 Protein, F3G5_4 Protein, adenosine kinase Protein, adk Protein, 2310026J05Rik Protein, 5033405D03Rik Protein, AI255373 Protein, AI987814 Protein, Ak Protein, adenosine kinase Protein, ATP:adenosine 5'-phosphotransferase Protein, Adenosine kinase Protein, adenosine kinase S homeolog Protein, ADK Protein, adk Protein, Adk Protein, CNN02130 Protein, LOC5572668 Protein, CMU_026370 Protein, adk.S Protein
- 背景
- Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 42.5 kDa (384aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_598840
- UniProt
- P55264
- 途径
- Ribonucleoside Biosynthetic Process
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