ADK Protein (AA 2-362) (His tag)
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- 抗原 See all ADK 蛋白
- ADK (Adenosine Kinase (ADK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-362
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宿主
- 人
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资源
- Insect Cells
- 标记
- This ADK protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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AAAEEEPKPK KLKVEAPQAL RENILFGMGN PLLDISAVVD KDFLDKYSLK PNDQILAEDK HKELFDELVK KFKVEYHAGG STQNSIKVAQ WMIQQPHKAA TFFGCIGIDK FGEILKRKAA EAHVDAHYYE QNEQPTGTCA ACITGDNRSL IANLAAANCY KKEKHLDLEK NWMLVEKARV CYIAGFFLTV SPESVLKVAH HASENNRIFT LNLSAPFISQ FYKESLMKVM PYVDILFGNE TEAATFAREQ GFETKDIKEI AKKTQALPKM NSKRQRIVIF TQGRDDTIMA TESEVTAFAV LDQDQKEIID TNGAGDAFVG GFLSQLVSDK PLTECIRAGH YAASIIIRRT GCTFPEKPDF H
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ADK Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ADK 蛋白
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Adenosine Kinase (ADK) (AA 22-362) protein (His tag)
ADK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Adenosine Kinase (ADK) (AA 1-345) protein (GST tag)ADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine Kinase (ADK) (AA 236-345) protein (GST tag)ADK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine Kinase (ADK) protein (GST tag,His tag)ADK 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Adenosine Kinase (ADK) (AA 1-361) protein (Strep Tag)Crystallography grade ADK 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ADK (Adenosine Kinase (ADK))
- 别名
- ADK (ADK 产品)
- 别名
- AK Protein, ADK Protein, DDBDRAFT_0186795 Protein, DDBDRAFT_0230174 Protein, DDB_0186795 Protein, DDB_0230174 Protein, ATPADK1 Protein, F3G5.4 Protein, F3G5_4 Protein, adenosine kinase Protein, adk Protein, 2310026J05Rik Protein, 5033405D03Rik Protein, AI255373 Protein, AI987814 Protein, Ak Protein, adenosine kinase Protein, ATP:adenosine 5'-phosphotransferase Protein, Adenosine kinase Protein, adenosine kinase S homeolog Protein, ADK Protein, adk Protein, Adk Protein, CNN02130 Protein, LOC5572668 Protein, CMU_026370 Protein, adk.S Protein
- 背景
- ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
- 分子量
- 41.4 kDa Including tag.
- UniProt
- P55263
- 途径
- Ribonucleoside Biosynthetic Process
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