PIN1 Protein (AA 1-165) (His tag)
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- 抗原 See all PIN1 蛋白
- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-165
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PIN1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMADEEKL PPGWEKRMSR SSGRVYYFNH ITNASQWERP SGGSTVGGSS KNGQGEPAKV RCSHLLVKHS QSRRPSSWRQ EKITRSKEEA LELINGYIQK IKSGEEDFES LASQFSDCSS AKARGDLGPF SRGQMQKPFE DASFALRTGE MSGPVFTDSG IHIILRTE
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
- Top Product
- Discover our top product PIN1 蛋白
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Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) protein
PIN1 宿主: 人 宿主: 大肠杆菌（E. Coli） 95 % by SDS - PAGE SDS
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-163) protein (His tag)PIN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-163) (Active) proteinPIN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-163) protein (GST tag)PIN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 64-163) protein (GST tag)PIN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 1-165) protein (Strep Tag)Crystallography grade PIN1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
- 别名
- Pin1 (PIN1 产品)
- 别名
- DOD Protein, UBL5 Protein, dod Protein, ubl5 Protein, pin1 Protein, pin1a Protein, 0610025L01Rik Protein, D9Bwg1161e Protein, pin1b Protein, zgc:73206 Protein, PPIase pin1 Protein, MdPin1 Protein, Pin1 Protein, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 Protein, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 S homeolog Protein, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 Protein, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 L homeolog Protein, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Protein, peptidyl-prolyl cis-trans isomerase Pin1 Protein, PIN1 Protein, Pin1 Protein, pin1 Protein, pin1.S Protein, pin1.L Protein, ppiD Protein, PITG_11123 Protein, LOC103419188 Protein
- 背景
- Pin1, also known as peptidyl-prolyl cis-trans isomerase NIMA-interacting1, is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. Recombinant mouse PIN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- 分子量
- 20.8 kDa (188aa) Confirmed by MALDI-TOF
- NCBI登录号
- NP_075860
- UniProt
- Q9QUR7
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